Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

256 – 270 of 3,304 results

256

HEPES Free Acid, >99%, Molecular Biology Reagent, MP Biomedicals™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid,N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid,N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

257

Thermo Scientific Chemicals Hanks' Balanced Salt Solution (10X), with calcium, with magnesium, with phenol red

Pricing & Availability
Specifications

Color	Red
Physical Form	Liquid
Recommended Storage	Ambient temperatures

258

MilliporeSigma™ TE Buffer Solution, pH 8.0, Calbiochem™,

Pricing & Availability

259

MP Biomedicals™ 1X Hanks' Balanced Salt Solution, Modified

Red, Clear Solution

Pricing & Availability

260

Gibco™ HBSS, calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing & Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	6.7 - 7.8
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Hanks' Balanced Salt Solution (HBSS)
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	270 - 305 mOsm/kg
Sterility	Sterile-filtered
Solution Type	Hank's Balanced Salt
Product Line	Gibco

261

HEPES sodium salt, ≥99%, MP Biomedicals™

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt,N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	2724248
CAS	75277-39-3
Molecular Weight (g/mol)	260.28
ChEBI	CHEBI:46758
MDL Number	MFCD00036463
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt,N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

262

MOPS, 99%

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

263

TRIS, MP Biomedicals™

TRIS, Molecular Biology Reagent, ≥99.95%, White Crystalline Powder

Pricing & Availability
Specifications

Storage Note 1	Store at Room Temperature (15-30°C). Store dessicated.
Molecular Weight (g/mol)	121.14
Loss on Drying	≤1%
Color	White
Grade	Molecular Biology Reagent
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
CAS	77-86-1
MDL Number	MFCD00004679
Solubility Information	Soluble in water (40% aq soln) Clear, Colorless Solution (550mg/mL), ethyleneglycol (79.1mg/mL), methanol (26mg/mL), anhydrous ethanol (14.6mg/mL), 95% ethanol (22.0mg/mL), DMF (14mg/mL), acetone (2mg/mL), ethyl acetate (0.5mg/mL), olive oil (0.4mg/mL), and chloroform (0.05mg/mL).
pH	10 to 11.5 (5% aq. soln.)
Synonym	Trometamol,THAM,Tromethamine,Tris base
Purity Grade Notes	≥99.95%
RTECS Number	TY2900000
Beilstein	741883
Molecular Formula	C4H11NO3
Melting Point	167°C to 172°C

264

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 6.5, 0.2 micron filtered

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

265

Tris Buffer, 0.5 L, MilliporeSigma™

Pricing & Availability

266

TE buffer, (20X, pH 7.6), RNAse free

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00236359 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
MDL Number	MFCD00236359
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

267

Physical Form	Liquid
pH	6.5
Chemical Name or Material	MNE buffer
TSCA	Yes
Recommended Storage	Ambient temperatures

268

Thermo Scientific Chemicals TRIS-glycine-native running buffer (10X), pH 8.5

Pricing & Availability
Specifications

Packaging	Plastic bottle
Physical Form	Liquid
Chemical Name or Material	TRIS-glycine-native running buffer (10X)
TSCA	Yes
Recommended Storage	Ambient temperatures

269

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Molecular Biology Grade, 99.9%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	TRIS
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

270

BES, 0.5M buffer soln., pH 6.0, Thermo Scientific™

CAS: 66992-27-6 Molecular Formula: C6H14NNaO5S Molecular Weight (g/mol): 235.23 MDL Number: MFCD00067425 InChI Key: CFQLQLSIZOWFNV-UHFFFAOYSA-M PubChem CID: 23671640 IUPAC Name: sodium;2-[bis(2-hydroxyethyl)amino]ethanesulfonate SMILES: C(CO)N(CCO)CCS(=O)(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	23671640
CAS	66992-27-6
Molecular Weight (g/mol)	235.23
MDL Number	MFCD00067425
SMILES	C(CO)N(CCO)CCS(=O)(=O)[O-].[Na+]
IUPAC Name	sodium;2-[bis(2-hydroxyethyl)amino]ethanesulfonate
InChI Key	CFQLQLSIZOWFNV-UHFFFAOYSA-M
Molecular Formula	C6H14NNaO5S

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Life Science Buffers

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Gibco™ HBSS, calcium, magnesium, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ HBSS, calcium, magnesium, no phenol red