Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 6.5
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
TRIS hydrochloride, >99%, Cell culture reagent grade, MP Biomedicals™
Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00012590 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: TRIS HCl,Tris(hydroxymethyl)aminomethane hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| Molecular Weight (g/mol) | 157.594 |
| MDL Number | MFCD00012590 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| Synonym | TRIS HCl,Tris(hydroxymethyl)aminomethane hydrochloride |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
Thermo Scientific Chemicals MES, 0.2M buffer soln., pH 6.0
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.248 |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.2
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
TRIS, 0.5M buffer soln., pH 8.6, Thermo Scientific™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
| Name Note | non-reducing |
|---|---|
| Health Hazard 3 | GHS P Statement P280-P264-P305+P351+P338-P337+P313 Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. |
| Health Hazard 2 | GHS H Statement H319 Causes serious eye irritation. |
| Color | Blue |
| Physical Form | Liquid |
| Health Hazard 1 | H335 |
| Chemical Name or Material | Tricine-SDS Sample Buffer |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Concentration | 2X |
Thermo Scientific Chemicals MOPS, 0.5M buffer soln., pH 7.5
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC Name | 3-morpholin-4-ylpropane-1-sulfonic acid |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Enzyme storage buffer in PBS and glycerol, autoclaved, Thermo Scientific™
For frozen storage of enzymes
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Enzyme storage buffer in PBS and glycerol |
| Recommended Storage | Ambient temperatures |
| Health Hazard 3 | P264b-P280i-P305+P351+P338 |
|---|---|
| Health Hazard 2 | GHS H Statement H319 Causes serious eye irritation. |
| Color | Colorless |
| Physical Form | Liquid |
| Health Hazard 1 | H319 |
| Chemical Name or Material | HEPES protein extraction buffer |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Concentration | 5X |
Thermo Scientific Chemicals PIPES, 0.5M buffer soln., pH 7.5
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 6.5
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.248 |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Gibco™ PBS (10X), pH 7.4
Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.
| Includes | None |
|---|---|
| Color | Clear, colorless solution |
| Physical Form | Liquid |
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Concentration | 10X |
| Osmolality | 2800 to 3100 mOsm/kg |
| Sterility | Sterile-filtered |
| Name Note | low endotoxin |
|---|---|
| Storage Note 1 | 2° to 8°C |
| Color | Colorless |
| Physical Form | Liquid |
| Chemical Name or Material | BES-buffered saline (2X) |
| TSCA | No |
| Recommended Storage | Keep cold |
| Name Note | low endotoxin |
|---|---|
| MDL Number | MFCD00132476 |
| Color | Colorless |
| Physical Form | Liquid |
| Chemical Name or Material | TRIS-buffered saline (10X, pH 7.6) |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Color | Red |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient temperatures |